LOCUS       sequence-97150-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LG6
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggctctac cacacctctg ttagcaaatt ctttgtctgt
      121 tcaccagttg gctgctcagg gagagatgct ctatctggct actcgtatcg aacaagaaaa
      181 tgttatcaat cacacggatg aagaaggatt tactcctctg atgtgggctg cagcacacgg
      241 gcaaatagct gtggtagagt tcctacttca gaatggtgct gatccccaac ttttaggaaa
      301 aggtcgagaa agtgcactgt cgttggcctg tagtaaaggc tacacagata ttgtcaaaat
      361 gctgcttgat tgtggagttg atgtaaatga atatgattgg aatggaggaa cacctctgct
      421 ttatgctgta catggaaatc atgtgaaatg tgtaaagatg ctcttagaaa gtggggctga
      481 tccaacaatt gaaactgact ctggatataa ttctatggat ctagctgtag ccctaggcta
      541 tagaagtgtt caacaggtta ttgagtcaca tttgttgaag ctgcttcaaa atatcaagga
      601 gtgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taa
//
