LOCUS       sequence-97151-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LG6
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(582..602)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(615..632)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             642..664
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    679..703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(703..722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(704..722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttcgt
       61 catcactcct tgatattttg aagcagcttc aacaaatgtg actcaataac ctgttgaaca
      121 cttctatagc ctagggctac agctagatcc atagaattat atccagagtc agtttcaatt
      181 gttggatcag ccccactttc taagagcatc tttacacatt tcacatgatt tccatgtaca
      241 gcataaagca gaggtgttcc tccattccaa tcatattcat ttacatcaac tccacaatca
      301 agcagcattt tgacaatatc tgtgtagcct ttactacagg ccaacgacag tgcactttct
      361 cgaccttttc ctaaaagttg gggatcagca ccattctgaa gtaggaactc taccacagct
      421 atttgcccgt gtgctgcagc ccacatcaga ggagtaaatc cttcttcatc cgtgtgattg
      481 ataacatttt cttgttcgat acgagtagcc agatagagca tctctccctg agcagccaac
      541 tggtgaacag acaaagaatt tgctaacaga ggtgtggtag agccctggaa atacaagttt
      601 tctctgccgc tgctgtgatg atgatgatga tgcatggtat atctccttct taaagttaaa
      661 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      721 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgt
//
