LOCUS       sequence-97155-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4MTL
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcggtggct ggttagaggc tgagaagaag ggggctccgc
      121 agaaagacag caccggggga gtcctagaag aatccaacaa gatagaacca tctcttcata
      181 gcctccagaa atttgttcct acagattacg ccagctacac tcaggagcat tatcggtttg
      241 caggaaagga gattgtcatc caggaatcca tagagagtta cggagcggtg gtgtggccag
      301 gggctatggc tttgtgtcaa tacttggagg aacatgccga ggaattgaat ttccaagatg
      361 caaaaatact tgaaattggt gccggaccag gccttgtttc cattgtggcc agtattttag
      421 gagctcaagt cacagcaaca gatttgcctg atgtcctggg aaaccttcaa tacaatcttt
      481 taaaaaacac actacaatgt acagcacatc tgcctgaagt gaaagaactg gtatgggggg
      541 aagacctgga caaaaacttt cccaagtcag ctttttacta tgattacgtc ctagcctcag
      601 atgtggtcta ccatcactac ttcctggaca agctgctcac caccatggtg tacctttccc
      661 agccagggac ggtgctgctt tgggcaaaca aattcaggtt cagcaccgac tatgaa
//
