LOCUS       sequence-97159-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4MVT
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcgaggtgg acatgcaccc ccctctgccc cagcctgtgc
      121 accctgatgt caccatgaaa ccattgccct tctatgaagt ctatggggag ctcatccggc
      181 ccaccaccct tgcatccact tctagccagc ggtttgagga agcgcacttt acctttgccc
      241 tcacacccca gcaagtgcag cagattctta catccagaga ggttctgcca ggagccaaat
      301 gtgattatac catacaggtg cagctaaggt tctgtctctg tgagaccagc tgcccccagg
      361 aagattattt tccccccaac ctctttgtca aggtcaatgg gaaactgtgc cccctgccgg
      421 gttaccttcc cccaaccaag aatggggccg agcccaagag gcccagccgc cccatcaaca
      481 tcacacccct ggctcgactc tcagccactg ttcccaacac cattgtggtc aattggtcat
      541 ctgagttcgg acggaattac tccttgtctg tgtacctggt gaggcagttg actgcaggaa
      601 cccttctaca aaaactcaga gcaaagggta tccggaaccc agaccactcg cgggcactga
      661 tcaaggagaa attgactgct gaccctgaca gtgaggtggc cactacaagt ctccgggtgt
      721 cactcatgtg cccgctaggg aagatgcgcc tgactgtccc ttgtcgtgcc ctcacctgcg
      781 cccacctgca gagcttcgat gctg
//
