LOCUS       sequence-97161-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4MX2
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(115..126)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcatcagt caagacagcc ccctctactc gctctctccc
      121 ctcgatggtc gctacaagcg tgacacgacc ccgctgcgcg cctacttctc cgagtacgcg
      181 ttattcaagt atcgcgtgca ggtggaggtg ctgtacttcg aagcgctgtg caaggaggtg
      241 ccggcgatca cgcagcttcg tggtgtcacc gatgcgcagc tgggggagct gcgggcgacc
      301 actttcgaga acttcgcagt cgacgatgca aaaataataa agggcattga ggcggtcacg
      361 aaccacgaca tcaaggccgt cgagtactac ctgaaggaca agatgtccgc ctgcggcctc
      421 gaggccgaaa aggagttcat ccactttggg ctaacgtcgc aggacatcaa caacacctcg
      481 attccgatgc tcctgcgcga cgctctgcac caccactaca ttccaacgct ggatcagctg
      541 atcgcgcttc tcaagagcaa gctgcctgaa tgggatgtcc cgatgctggc gcggacgcac
      601 ggccaaccgg cgagcccgac gaacctggcg aaagagttca tggtatggat tgagcggctg
      661 gaagagcagc ggacgatgct gctgagcatc ccaaacacag gcaagttcgg cggcgctacc
      721 ggcaacttca acgcgcacct ctgtgcgtac cctggcgtga actggctgga ac
//
