LOCUS       sequence-97164-e         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An LV packaging vector expressing eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  TITLE     Lentiviral vectors for stable ectopic expression or knockdown of WNT
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOTTC155
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    133..157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(246..267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(507..526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta gaggatccac tagtccagtg tggtggaatt
      121 ctgcagatat caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      181 tggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      301 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttc
//
