LOCUS       sequence-97166-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An LV packaging vector expressing eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  TITLE     Lentiviral vectors for stable ectopic expression or knockdown of WNT
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOTTC155
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(126..131,132..149,150..156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     169..191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     188..204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     188..204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     223..243
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        225..243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        271..497
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
       61 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      181 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
      241 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
      301 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      361 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      421 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      481 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      541 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      601 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      661 tgtgga
//
