LOCUS       sequence-97178-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JXT A
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(459..479)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(492..509)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             519..541
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    556..580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(580..599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(581..599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat cctcgagtgc ggccgcaagc ttcgtcatca cttcctaggc
       61 ttcttatcac catacagagc ttgtttaagt tgttctaata catcattttc ataaacagac
      121 ctttcttccc aaatagataa cactcttcca aggtgcttct tacaactttc atcagtttca
      181 cttgaaacat gcttaaaagc ctccactata actggtgcaa aatcttttgt aaactctggc
      241 cccttcctct tgctgttctg tatgacatca ttggctaggt agagaaaagt aagcttcctg
      301 tttggtttgg ctttccgcag ctcccgctcc cacacggtga cgatgggccg cgagtgttta
      361 cggtggtgaa tgagccacag ggacaaggtc tgcacgctct gctgcgagtt gctcaactcc
      421 gacagcttct tctccagcgc cgcctcagag aaggctgagc cctggaaata caagttttct
      481 ctgccgctgc tgtgatgatg atgatgatgc atggtatatc tccttcttaa agttaaacaa
      541 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      601 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      661 gccac
//
