LOCUS       sequence-97180-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JXT A
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(573..591)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttta actttaagaa ggagatatac catgtcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggctcag ccttctctga ggcggcgctg gagaagaagc
      121 tgtcggagtt gagcaactcg cagcagagcg tgcagacctt gtccctgtgg ctcattcacc
      181 accgtaaaca ctcgcggccc atcgtcaccg tgtgggagcg ggagctgcgg aaagccaaac
      241 caaacaggaa gcttactttt ctctacctag ccaatgatgt catacagaac agcaagagga
      301 aggggccaga gtttacaaaa gattttgcac cagttatagt ggaggctttt aagcatgttt
      361 caagtgaaac tgatgaaagt tgtaagaagc accttggaag agtgttatct atttgggaag
      421 aaaggtctgt ttatgaaaat gatgtattag aacaacttaa acaagctctg tatggtgata
      481 agaagcctag gaagtgatga cgaagcttgc ggccgcactc gaggatccgg ctgctaacaa
      541 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      601 tg
//
