LOCUS       sequence-97181-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JXT B
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(564..582)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      578..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggctcctcct tctctgagtc ggcgctggag aagaagctct
      121 cggagctgag caactctcag cacagcgtgc agaccctgtc cctttggctc atccaccacc
      181 gcaagcacgc gggacccatc gtctccgtgt ggcaccgcga gctccgcaaa gccaaatcaa
      241 atagaaagct tacttttctg tatttagcga atgatgtcat ccaaaacagt aaaaggaaag
      301 gacctgaatt cactagagaa tttgaatctg tccttgtgga tgctttttct catgttgcca
      361 gagaggcaga tgaaggctgt aaaaaacctt tagaaagatt gctgaacatc tggcaagaac
      421 gaagtgtgta tggcggcgag ttcatacagc agctgaagct gtctatggag gactccaaga
      481 gcccttgatg acgaagcttg cggccgcact cgaggatccg gctgctaaca aagcccgaaa
      541 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag
      661 aggcc
//
