LOCUS       sequence-97182-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JXT B
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(451..471)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(484..501)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             511..533
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    548..572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(572..591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(573..591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttgttagc agccggatcc tcgagtgcgg ccgcaagctt cgtcatcaag ggctcttgga
       61 gtcctccata gacagcttca gctgctgtat gaactcgccg ccatacacac ttcgttcttg
      121 ccagatgttc agcaatcttt ctaaaggttt tttacagcct tcatctgcct ctctggcaac
      181 atgagaaaaa gcatccacaa ggacagattc aaattctcta gtgaattcag gtcctttcct
      241 tttactgttt tggatgacat cattcgctaa atacagaaaa gtaagctttc tatttgattt
      301 ggctttgcgg agctcgcggt gccacacgga gacgatgggt cccgcgtgct tgcggtggtg
      361 gatgagccaa agggacaggg tctgcacgct gtgctgagag ttgctcagct ccgagagctt
      421 cttctccagc gccgactcag agaaggagga gccctggaaa tacaagtttt ctctgccgct
      481 gctgtgatga tgatgatgat gcatggtata tctccttctt aaagttaaac aaaattattt
      541 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      601 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      661 tgcggttgct ggcgcctata tcgccgacat c
//
