LOCUS       sequence-97184-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LAMP1-phLuorin-N-8
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(231..251)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     846..874
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actccttgat gatggccatg ttattctcct cgcccttgct caccatggtg gcgaccggtc
       61 cagaccctga gatggtctga tagccggcgt gactcctctt cctgccaatg aggtaggcaa
      121 tgaggacgat gaggaccagc cctgccaggg caccgcccac agcaatgggg atcaacatgt
      181 tgttggatcc tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
      241 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagagta gaagttgtaa
      301 acaggtaatg gttgtctggt aaaaggacgg gcccatcgcc aattggagta ttttgttgat
      361 aatggtctgc tagttgaacg cctccatctt caatgttgtg tctaattttg aagttagctt
      421 tgattccatt cttttgtttg tctgccatga tgtacacctg gtgatcgtta tagttgtatt
      481 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
      541 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
      601 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
      661 catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa gttaaagtag
      721 tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa
      781 gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
      841 catcaccatc taattcaaca agaattggga caactccagt gaaa
//
