LOCUS       sequence-97188-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3CEG
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcgcaaatc aggaaaaaaa actgggtgaa tactccaaga
      121 aggcggctat gaaacccaaa cctttgtcag tattaaagtc acttgaagaa aaatatgtgg
      181 ctgttatgaa gaaattacag tttgatacgt ttgaaatggt ttctgaagat gaagatggga
      241 aattgggatt taaagtaaat taccactaca tgtctcaggt gaaaaatgct aatgatgcga
      301 acagtgctgc cagagctcgc cgccttgccc aggaagctgt gacactttca acctcactgc
      361 ctctgtcttc atcctctagt gtgtttgtac gctgtgatga ggagcgactt gatatcatga
      421 aggttctaat aactggtcca gcggacaccc cttatgcaaa tggctgcttt gagtttgatg
      481 tgtattttcc tcaagattat cccagttcac cccctcttgt gaatctagag acaactggtg
      541 gtcatagcgt gcgattcaat ccaaaccttt ataatgatgg caaggtttgt ttaagcatct
      601 taaacacgtg gcatggaaga ccagaagaga agtggaatcc tcagacctca agctttttgc
      661 aagtgttggt gtctgtccag tcccttatat tagtagctga gccttatttt aatgaaccgg
      721 gatatgaacg gtctagaggc actcccagtg gcacacagag ttctcgagaa tatgatgg
//
