LOCUS       sequence-97189-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2P0E
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             680..697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(750..768)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggttccgcgt ggtagtaaaa catttatcat tggaatcagt ggtgtgacaa
      121 acagtggcaa aacaacactg gctaagaatt tgcagaaaca cctcccaaat tgcagtgtca
      181 tatctcagga tgatttcttc aagccagagt ctgagataga gacagataaa aatggatttt
      241 tgcagtacga tgtgcttgaa gcacttaaca tggaaaaaat gatgtcagcc atttcctgct
      301 ggatggaaag cgcaagacac tctgtggtat caacagacca ggaaagtgct gaggaaattc
      361 ccattttaat catcgaaggt tttcttcttt ttaattataa gccccttgac actatatgga
      421 atagaagcta tttcctgacg attccatatg aagaatgtaa aaggaggagg agtacaaggg
      481 tctatcagcc tccagactct ccgggatact ttgatggcca tgtgtggccc atgtatctaa
      541 agtacagaca agaaatgcag gacatcacat gggaagttgt gtacctggat ggaacaaaat
      601 ctgaagagga cctctttttg caagtatatg aagatctaat acaagaacta tgatgacgaa
      661 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      721 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aa
//
