LOCUS       sequence-97190-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2P0E
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(634..651)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(661..678)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tcgtcatcat agttcttgta ttagatcttc atatacttgc aaaaagaggt cctcttcaga
      121 ttttgttcca tccaggtaca caacttccca tgtgatgtcc tgcatttctt gtctgtactt
      181 tagatacatg ggccacacat ggccatcaaa gtatcccgga gagtctggag gctgatagac
      241 ccttgtactc ctcctccttt tacattcttc atatggaatc gtcaggaaat agcttctatt
      301 ccatatagtg tcaaggggct tataattaaa aagaagaaaa ccttcgatga ttaaaatggg
      361 aatttcctca gcactttcct ggtctgttga taccacagag tgtcttgcgc tttccatcca
      421 gcaggaaatg gctgacatca ttttttccat gttaagtgct tcaagcacat cgtactgcaa
      481 aaatccattt ttatctgtct ctatctcaga ctctggcttg aagaaatcat cctgagatat
      541 gacactgcaa tttgggaggt gtttctgcaa attcttagcc agtgttgttt tgccactgtt
      601 tgtcacacca ctgattccaa tgataaatgt tttactacca cgcggaacca ggccgctgct
      661 gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag tt
//
