LOCUS       sequence-97192-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2QQ8
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcaacgctg tgctcacctg gaataatgag atcttaccta
      121 actgggaaac aatgtggtgc tctagaaaag ttcgagattt atggtggcag ggaatccctc
      181 caagtgtgag aggcaaagtc tggagcttag ccattggcaa cgagttaaat atcacccacg
      241 agctctttga catctgtctt gcccgagcca aggagaggtg gcggtccctt agcacaggag
      301 gctctgaagt ggagaacgaa gatgctggtt tttcagcagc agacagagaa gccagtctgg
      361 agcttattaa actggacatt tctagaacat ttcctaatct ctgcattttc cagcaaggtg
      421 gtccatatca tgacatgttg cacagtattt tgggcgctta tacttgttac cggccagatg
      481 tgggttatgt ccagggcatg tccttcatag cagcagtgtt gatcttgaac ttagatactg
      541 cagatgcctt tattgccttt tctaaccttc tgaataaacc ctgtcaaatg gcgtttttta
      601 gagtggacca tggccttatg ttgacttatt ttgctgcatt tgaagtattc tttgaagaaa
      661 atttgccgaa attatttgcg catttcaaga agaacaacct aactccagat atctacctaa
      721 ttgattggat ctttacctta tatagt
//
