LOCUS       sequence-97193-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             141..197
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             198..218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag
       61 atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg
      121 aacaagctag cggaagcgga gccactaact tctccctgtt gaaacaagca ggggatgtcg
      181 aagagaatcc cgggccaccc aagaagaaga ggaaggtgtc caatctcctg actgttcacc
      241 agaacctccc tgcgctgcca gtagatgcca ctagcgatga ggtcaggaaa aatctcatgg
      301 atatgtttag ggatagacag gcgttttctg aacacacctg gaaaatgctg cttagcgtgt
      361 gccgatcctg ggcagcctgg tgtaagctga acaatcgcaa atggttcccc gccgagccgg
      421 aggacgtgcg cgattacctg ctgtatctcc aggcaagagg gctggctgtc aagactatcc
      481 agcagcactt gggccaactg aatatgctgc atcgacgcag cgggctcccc cggcctagcg
      541 attcaaacgc agtctccctt gttatgagga gaatt
//
