LOCUS       sequence-97194-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3BFN
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggccctccag ctcgcgtaag ggtggctgtg cgactgcggc
      121 catttgtgga tggaacagcg ggagcaagtg atcccccctg tgtgcggggc atggacagct
      181 gctctctaga gattgctaac tggaggaacc accaggagac tctcaaatac cagtttgatg
      241 ccttctatgg ggagaggagt actcagcagg acatctatgc aggttcagtg cagcccatcc
      301 taaggcactt gctggaaggg cagaatgcca gtgtgcttgc ctatggaccc acaggagctg
      361 ggaagacgca cacaatgctg ggcagcccag agcaacctgg ggtgatcccg cgggctctca
      421 tggacctcct gcagctcaca agggaggagg gtgccgaggg ccggccatgg gccctttctg
      481 tcaccatgtc ttacctagag atctaccagg agaaggtatt agacctcctg gaccctgctt
      541 cgggagacct ggtaatccga gaagactgcc gggggaatat cctgattccg ggtctctccc
      601 agaagcccat cagtagcttt gctgattttg agcggcactt cctgccagcc agtcgaaatc
      661 ggactgtagg agccacccgg ctcaaccagc gctcctcccg cagtcatgct gtgctcctgg
      721 tcaaggtgga ccagcggg
//
