LOCUS       sequence-97196-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2OQK
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(513..531)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      527..574
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcatgccga agaataaagg aaaaggaggt aaaaatcgta
      121 ggagagggaa aaatgacagt gagggagaca agagagagtt agtattcaaa gaggaagggc
      181 aagaatatgg ccaagtccaa agaatgttgg gcaacggacg acttgatgcg tattgctttg
      241 atggacagaa acgtctttgt catattaggg gcaagatgag aaagaaagtt tgggtaaatc
      301 ctggcgatat tgtattagtt tcgctcagag actttcaaga ctcaaagggc gatattattc
      361 tcaagtatac tccagatgag gctagagcat tgaaatctaa aggtgaaatt cctgaaacta
      421 caaagatcaa tgagtgatga cgaagcttgc ggccgcactc gaggatccgg ctgctaacaa
      481 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      541 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
      601 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      661 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      721 tagc
//
