LOCUS       sequence-97197-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2OQK
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(396..416)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(429..446)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             456..478
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    493..517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(517..536)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(518..536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatcctcga gtgcggccgc aagcttcgtc atcactcatt gatctttgta
       61 gtttcaggaa tttcaccttt agatttcaat gctctagcct catctggagt atacttgaga
      121 ataatatcgc cctttgagtc ttgaaagtct ctgagcgaaa ctaatacaat atcgccagga
      181 tttacccaaa ctttctttct catcttgccc ctaatatgac aaagacgttt ctgtccatca
      241 aagcaatacg catcaagtcg tccgttgccc aacattcttt ggacttggcc atattcttgc
      301 ccttcctctt tgaatactaa ctctctcttg tctccctcac tgtcattttt ccctctccta
      361 cgatttttac ctccttttcc tttattcttc ggcatgccct ggaaatacaa gttttctctg
      421 ccgctgctgt gatgatgatg atgatgcatg gtatatctcc ttcttaaagt taaacaaaat
      481 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      541 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      601 acaggtgcgg ttgctggcgc ctatatcg
//
