LOCUS       sequence-97198-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase and Renilla luciferase from the EFS 
            promoter and one U6-driven sgRNA targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: 
            AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..120)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
       61 ttgattatcg ataagcttga tatcgaattc ttaagcgtaa tctggaacat cgtatgggta
      121 gtcaccatct tcgagcagtc tcaccatggc cccggtttca gagtcaagat tgcggatata
      181 gttcataacg atgttcacat ttgtccaacc tccagcttgc ataatctctg gtatggagac
      241 tcctgcccgc gccatgtctc gtgcggcccc caccctagca gaatgacctg accaggcgag
      301 atacctctgg ccactgtcat ccttggctcc gtatatgagt cggtgggttg cctcaaagat
      361 cccttcaagg gcccgtgtac tcaattggct ggtggcagag ggcgcggcga ctccgttttt
      421 tctcactcga cagaacaagt aattattagg gtcgtcagcc acgccggaca ctgagatcca
      481 tctttccacc agcttcgtta cgcccaggga caaggccttt tcaacgccag cggtgctgac
      541 caatgtcttt gtgcgtccaa tatgaataag catccggccg ccgtccgttc gactgatgtc
      601 tttcactctg atccgggcga tctcagctat gcgcagaagt gtgttatatg caattcccag
      661 aaatgcgagg ttcctgatgt cctggcaccg atcgctgttc tccatcaggg atctgacctg
      721 gtcgaaatca gtccgctcaa aagcgagagc ctgcttagcc ctctcacccg catctacgtt
      781 ttcctttcta attctcctca taacaaggga gactgcgt
//
