LOCUS       sequence-97199-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2P65
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(611..631)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(644..661)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atcctcgagt gcggccgcaa gcttcgtcat caacttggtt
       61 gttccactag tatttgttgg aacctcctct ccagggcttt gtccttctct ataaactgcc
      121 tatactcact tacagtcgtt gccccgatgc atctgagctc tcctcttgcg agcataggtt
      181 tgaggatgtt ccctgcatct agtgcccctt cagccactgc acctgccccg actacagtgt
      241 gaatctcatc gataaacatg acgacttgtc cttctgcgtc ttgcacttct ttcaaaatgg
      301 atttcagtct ctcttcaaaa tctcctctgt acttcgctcc cgcgatgaga gaggacagat
      361 ctagagatac taacttgcgc ccctttaacg aatctggcac atccccctgg acaattttga
      421 tggccaatcc ttcgacgatg gccgtctttc caaccccagg gtctcccaaa agaatgggat
      481 tgttctttgt tcttcgggac aagatctgga tggctctcct aatttctgtg tctctcccaa
      541 taacaggatc tagcttccct gctcttgcca aggcagtgag atctcggctg tatttttcca
      601 acgcttggta gccctggaaa tacaagtttt ctctgccgct gctgtgatga tgatgatgat
      661 ggctgctgcc catggtatat ctccttctta aagtt
//
