LOCUS       sequence-97200-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKT230 derivative; PBAD_rfp; cmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Bi C, Su P, Muller J, Yeh YC, Chhabra SR, Beller HR, Singer SW, 
            Hillson NJ
  TITLE     Development of a broad-host synthetic biology toolbox for Ralstonia 
            eutropha and its application to engineering hydrocarbon biofuel 
            production.
  JOURNAL   Microb Cell Fact. 2013 Nov 13;12:107. doi: 10.1186/1475-2859-12-107.
  PUBMED    24219429
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-11-13"; volume: "12:107. doi"; pages: " 
            10.1186/1475-2859-12-107"
COMMENT     Sequence Label: pKTrfp
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      147..218
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      234..261
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
       61 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc
      121 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      181 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      241 cttcgggtgg gcctttctgc gtttatacct agggccctga aagcgaccag gtgctcggcg
      301 tggcaagact cgcagcgaac ccgtagaaag ccatgctcca gccgcccgca ttggagaaat
      361 tcttcaaatt cccgttgcac atagcccggc aattcctttc cctgctctgc cataagcgca
      421 gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc gggtcggtgg
      481 ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac atgaggcatg
      541 ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga aagttctttt
      601 accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg tcactcccgt
      661 actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac tgtcacgaac
      721 ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg ggctggcggg
      781 gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg gttatcagcg
      841 cccttgtggg gcgctgctgc c
//
