LOCUS       sequence-97204-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKT230 derivative; PBAD_rfp; cmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Bi C, Su P, Muller J, Yeh YC, Chhabra SR, Beller HR, Singer SW, 
            Hillson NJ
  TITLE     Development of a broad-host synthetic biology toolbox for Ralstonia 
            eutropha and its application to engineering hydrocarbon biofuel 
            production.
  JOURNAL   Microb Cell Fact. 2013 Nov 13;12:107. doi: 10.1186/1475-2859-12-107.
  PUBMED    24219429
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-11-13"; volume: "12:107. doi"; pages: " 
            10.1186/1475-2859-12-107"
COMMENT     Sequence Label: pKTrfp
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(156..440)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(214..233)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa
       61 ctttgaaacg catgaactct ttgataacgt cttcgctact cgccatatgt atatctcctt
      121 cttaaaagat cttttgaatt cccaaaaaaa cgggtatgga gaaacagtag agagttgcga
      181 taaaaagcgt caggtagaat ccgctaatct tatggataaa aatgctatgg catagcaaag
      241 tgtgacgccg tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt
      301 acgcgttttt gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt
      361 taccggtttg gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca
      421 atatggacaa ttggtttctt ctctgaatgg cgggagtatg aaaagtatgg ctgaagcgca
      481 aaatgatccc ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc
      541 gattgaggcc aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
      601 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
      661 ttgccgaccg gg
//
