LOCUS       sequence-97230-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) 
            cDNA allele B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   McBride CS, Baier F, Omondi AB, Spitzer SA, Lutomiah J, Sang R, 
            Ignell R, Vosshall LB
  TITLE     Evolution of mosquito preference for humans linked to an odorant 
            receptor.
  JOURNAL   Nature. 2014 Nov 13;515(7526):222-7. doi: 10.1038/nature13964.
  PUBMED    25391959
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-11-13- 13"; volume: "515"; issue: "7526"; 
            pages: "222-7"
COMMENT     Sequence Label: pUASTattb AaegOr4-B
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    143..176
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    209..303
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        322..560
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     507..528
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt
       61 atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg
      121 atttcactgg aactaggcta gcataacttc gtataatgta tgctatacga agttatgcta
      181 gcggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc
      241 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      301 gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
      361 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
      421 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
      481 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      541 aatacaagaa gagaactctg aatagggaat tgggaattca tgactcaatc ccttgagttt
      601 gaccaaacct ttggcttcat cgccaaggtc ttgcaaatga tcggttatcc aagctgttta
      661 gcaccgtacc caacgacctt cgccagtcga ctcaagtcca gttccggttt cgtg
//
