LOCUS       sequence-97235-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2PLU
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(556..576)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(589..606)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             616..638
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    653..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(677..696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tcctcgagtg cggccgcaag cttcgtcatc aaactccaga
       61 agcagtgatg catacagact tggttggctt tccattttga gaaccacact tctcaatgct
      121 ctttactact tccattcctt ccaaaacctt tccaaataca acatgctttc cgtccaacca
      181 tgggcatgga acagttgtaa tgaagaattg ggatccatta gtgtttggac cagcattagc
      241 catagagaga aggaatgggg cgtcatgagt gtagacgaag ttttcatctc tgaacttgga
      301 gccatagatt gactcaccac cagtaccatc accacgagta aaatcaccac cttggatcat
      361 gaattgtgga atgatacgat ggaagaatga tccagtgtag caaaggggct ttcctgactg
      421 tcccattccc ttttctccag tgcagagtgc tctgaagttt tcggctgtta ttggtacttt
      481 atctgcaaac aactccatag tgatacgacc ggctggagtt tggccgattg agatatcaaa
      541 gtatacaact gggttgccct ggaaatacaa gttttctctg ccgctgctgt gatgatgatg
      601 atgatgcatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      661 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      721 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      781 ctatatcg
//
