LOCUS       sequence-97236-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ER-3
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(187..894)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(462..481)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     731..749
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
       61 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt
      121 tatctagatc cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cttacagctc
      181 gtccttcttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
      241 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
      301 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
      361 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
      421 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
      481 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
      541 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      601 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
      661 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
      721 ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
      781 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
      841 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatcgacc
//
