LOCUS       sequence-97237-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4GM2
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(605..625)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(638..655)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             674..696
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    715..731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgggctttgt tagcagccgg atcctcgagt gcggccgcaa gcttcgtcat cattcctttt
       61 caagtatatg atcaattaat ttaaaatcta ctgcttcatc tgcattaaaa tatttatctc
      121 tttctaaaac atttgaaata acatttgtat ctttttctgt atttttagaa attatttcta
      181 ttactttttt tttggtgttc attatttctt tattttgtat ttcaatattg gttgcttggt
      241 taaagggtat gatagaataa gattgattta aacaaaaaga ggaatttttt aaagaaaagc
      301 gatagccttt ttttccactg ctagctaata tacaagcaat tccatacgct tttccaagac
      361 aataagtata cacatcagaa gatatataat taattacatc aactattgat attacatcag
      421 ttataccatt caaatttatt attttattat tatctatatc acctgtactg ttaatatata
      481 aatgtatagg ttttctttta gattcatatt ctaaatataa taactgtgat atgatttgtt
      541 ctgatatatg aggataaata ggtgatgata aaaatataat tcttttagat aaaagtaatg
      601 aagggccctg gaaatacaag ttttctctgc cgctgctgtg atgatgatga tgatggctgc
      661 tgcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      721 tccgctcaca att
//
