LOCUS       sequence-97241-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3TB2
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtatg ggatatcatt taggagcaac atttccaaat
      121 tttacagcca aagcttcagg tatagatggt gattttgaat tatataaata tatagaaaat
      181 agttgggcaa tattatttag tcatccaaat gattttactc cagtatgtac aactgagtta
      241 gcagaattag gaaaaatgca tgaagacttt ttaaaactta attgtaaatt aataggcttt
      301 agttgtaatt caaaagagtc acatgataaa tggatagaag atataaaata ttatggaaaa
      361 ttaaataaat gggaaattcc tattgtttgt gatgaatcac gagaacttgc aaataaatta
      421 aaaattatgg atgaacaaga aaaagatata actggtttac cattaacatg tagatgcctt
      481 ttttttattt ctcctgaaaa aaaaattaaa gcaactgtat tatatccagc aacaacaggt
      541 agaaatgctc atgaaatttt aagagtttta aaatctttac aacttactta taccacccca
      601 gtagctacac ctgttaattg gaatgaaggt gacaaatgtt gtgtcattcc aactcttcag
      661 gatgatgaaa tatctaaaca ttttaaaaat gaaataacaa aagttgaaat gccatcaaaa
      721 aaaaaatatc tcagatttgt taatttatga tgacgaagct tgcggccgca ctcgagca
//
