LOCUS       sequence-97244-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of tTA-EBFP fusion protein (tTA from 
            CL-CTIG, Addgene plasmid 14901).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: tTA_BFP
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(225..244)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccgccggc gaaaacctgt attttcagct gatgtctaga ttagataaaa gtaaagtgat
      181 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
      241 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
      301 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
      361 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
      421 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
      481 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
      541 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
      601 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
      661 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
      721 tgaattgatc atatgcggat tag
//
