LOCUS       sequence-97246-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of tTA-EBFP fusion protein (tTA from 
            CL-CTIG, Addgene plasmid 14901).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: tTA_BFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(18..731,732..734,735..737))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     671..692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gactcgagcg gccgctatta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
       61 gtgcggaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcacg
      121 gactgggtgc tcaggtagtg gctgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      181 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct ccacgttgtg gcggatcttg
      241 aagttcacct tgatgccgtt cttctgcttg acggccatga tatagatgtt gtggctgttg
      301 aagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gacgcccttc
      361 agctcgatgc ggttcactag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      421 gtgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      481 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg cacgccgtgg
      541 ctcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      601 ttcagggtca gcttgccgtt ggtggcatcg ccctcgccct cgcccctcac gctgaacttg
      661 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      721 tcgcccttgc tcaccataga gccaccaccg gagcctccgc cggtacctcc accgtactcg
      781 tcaattccaa gggcatcggt aaacatctgc tc
//
