LOCUS       sequence-97250-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of tTA-EBFP fusion protein (tTA from 
            CL-CTIG, Addgene plasmid 14901).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: tTA_BFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..432
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(508..529)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagccgc
       61 gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct cccggacgac
      121 gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc cgcgggacac
      181 acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga gctccactta
      241 gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg
      301 ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc
      361 gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac
      421 gagtacggtg gaggtaccgg cggaggctcc ggtggtggct ctatggtgag caagggcgag
      481 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgaggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
      601 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc
      661 cacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      721 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc
      781 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tagtgaaccg catcgagctg
//
