LOCUS       sequence-97257-e        5997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid contains the integrase gene under the control of the 
            arabinose-inducible promoter, Pbad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pIntegrase_10
FEATURES             Location/Qualifiers
     source          1..5997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..107
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(601..620)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             612..1427
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             612..1427
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(685..704)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1544..1561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      2135..2723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2624..2643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3205..3234)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(3281..4159)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4186..4470
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4393..4412
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
       61 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      121 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      181 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatttc actgacaccc
      241 tcatcagtgc caacatagta agccagtata cactccgcta gcgctgaggt ctgcctcgtg
      301 aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga
      361 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt
      421 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag
      481 caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg
      541 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
      601 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt
      661 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
      721 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
      781 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
      841 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
      901 tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag
      961 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1021 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1081 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1141 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     1201 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1261 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1321 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1381 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     1441 attggttgta acactggcag agcattacgc tgacttgacg ggactcgaga ttaccgcctt
     1501 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1561 ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga
     1621 caccttgccc gtttttttgc cggactgcag caatggcaac aacgttgcgc aaactattaa
     1681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2881 aggtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     2941 cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg
     3001 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccctcgagc cgtcaattgt
     3061 ctgattcgtt accaattata ctagctccgg caaaaaaacg ggcaaggtgt caccaccctg
     3121 ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt ctaagaaaag
     3181 gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg agccagtgag
     3241 ttgattgcta cgtaattagt tagttagccc ttagtgactc ttatgacaac ttgacggcta
     3301 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
     3361 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
     3421 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
     3481 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
     3541 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
     3601 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
     3661 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
     3721 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
     3781 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
     3841 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
     3901 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
     3961 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
     4021 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
     4081 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
     4141 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
     4201 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
     4261 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
     4321 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     4381 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
     4441 ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc tcaaacagga
     4501 agaaggctag atgatcacca ccaataaagt ggcgatttat gtgcgtgtta gtaccaccaa
     4561 tcaggttgaa gagggttata gcattgatga acagaaagat aaactgagca gctattgcga
     4621 tatcaaagat tggaacgtgt acaaagtgta taccgatggt ggttttagcg gtagcaatac
     4681 cgatcgtccg gcactggaaa gcctgattaa agatgcaaaa aaacgcaaat tcgataccgt
     4741 gctggtgtat aaactggatc gtctgagccg tagccagaaa gacaccctgc atctgattga
     4801 agatgtgttt atcaaaaatg gcattgaatt tctgagcctg caagagaatt ttgataccag
     4861 caccccgttt ggtaaagcaa tgattggtct gctgagcgtt tttgcacagc tggaacgtga
     4921 gcagattaaa gaacgtatgc agctgggtaa actgggtcgt gcaaaaagcg gtaaaagcat
     4981 gatgtgggca aaaaccagct atggctatga ttatcacaaa gaaaccggca ccgttaccat
     5041 taatccggca caggcactga ccatcaaatt tatctttgaa agctatctgc gtggtcgcag
     5101 cattaccaaa ctgcgtgatg atctgaatga gaaatatccg aaacatgtgc cgtggtcata
     5161 tcgtgcagtt cgtaccattc tggataatcc ggtttattgc ggtttcaacc agtataaagg
     5221 tgaaatctat ccgggtaacc atgaaccgat tattagcaaa gaagagtacg ataaaaccca
     5281 gagcgaactg aaaattcgtc agcgtaccgc agcagaaaat gttaatccgc gtccgtttca
     5341 ggccaaatat atcctgagcg gtattgccca gtgtggttat tgtggtgcac cgctgaaaat
     5401 catgctgggt gttaaacgta aagatggtag ccgtctgaaa aagtatgaat gtcatcagcg
     5461 tcatccgcgt accctgcgtg gtgttaccac ctataatgat aacaaaaaat gcgacagcgg
     5521 cttttactac aaagacaaac tggaagccta tgtgctgaaa gaaattagca aactgcagga
     5581 cgatgcagat tatctggata aaatctttag cggtgataac gccgaaacca ttgatcgtga
     5641 gagctataaa aagcagatcg aggaactgag caaaaaactg agtcgtctga acgatctgta
     5701 tatcgatgat cgtattaccc tggaagaact gcagagcaaa agcgcagaat ttatcagcat
     5761 gcgtggcacc ctggaaaccg aactggaaaa tgatcctgca ctgcgtaaaa acaaacggaa
     5821 agcagatatg cgtaaactgc tgaatgccga aaaagtgttc agcatggatt atgagagtca
     5881 gaaagttctg gttcgtcgcc tgatcaataa agttaaagtt accgctgaag atattgtgat
     5941 taattggaaa atctaacaga taaaaaaaat ccttagcttt cgctaaggat gatttct
//
