LOCUS       sequence-97259-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are 
            expressed as a single poly-cistronic unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-C5
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(322..375)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgattcctgg tgggttagcg agttggaaag gataaagtct aggtccagga ggtcgttgaa
       61 ctcctcggtc tccctccggg ctaggagggc cgggttgtta ctgctgcaag ctgcaccagc
      121 tccgccactc tccaggtctg tggccaccgt cgccgccagg tcgtaggggc ggccgggaag
      181 tgggggaagt cgcttcatgt gagagagttc ctcacgccaa cggttagtcg gggcacctgc
      241 tggacgcagt gtcttctccc ttcccgccgg gccggacgcg aacgtggaga aggacgggag
      301 cagagcgtcg ctgacagcca ttgggccagg attctcctcc acgtcaccgc atgttagaag
      361 acttcctctg ccctctcctc cggacccgcc gcccatgtgc gacaggggca gtgtgccgtt
      421 aatggccgtg ccgggcaccg ggccgctctg gtagtgctgg gccatgtgca gtctactggg
      481 cgcagcgggc tccggcacct cggcgccggg gaggtacatg ctgatcatgt cccggaggtc
      541 cccggcctgg cagggcgccc tggagtggga ggaagaggta accacggggg ggctggagct
      601 ggcctcggac ttgaccacag agcccatgga gcccagcgcc ataccggggg tgccctgctg
      661 cgagtaggac atgctgtagg tgggcgagcc gttcatgtag gtctgcgagc tggtcatgga
      721 gttgtactgc agggcgctga cgtcgtagcg gtgcatcggt tgcatctgtg ccgcgccgtg
      781 agcgttgagg cccgggtgct gcgggtagcc cagctgctcc tgcatcatgc tgtagctgcc
      841 gttgctccag ccgttcatgt gcgc
//
