LOCUS       sequence-97264-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with mCherry ectodomain, no linker domain, G 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: mCherry_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     294..313
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(387..407)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac
       61 tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa
      121 gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa
      181 gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc
      241 cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt
      301 ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc
      361 actttacttt tatctaatct agacataccc tgaaaataca ggttttcaac acaaagagcc
      421 actgtcacta gcaagccata acaaaacagg actccagcaa ccacgaccag tgcccaaaaa
      481 agcttcttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      541 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      601 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      661 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      721 tcggggtaca tccgctcgga ggaggcct
//
