LOCUS       sequence-97266-e        3759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An array constructed by concatenating the attB/P sites of the 11 
            phage integrases and can be used to distinguish 2048 possible 
            combinations of events..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pMemoryArray
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(2604..2621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2738..3282)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2818..2837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3367..3461)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3482..3759,1..382))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
       61 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
      121 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
      181 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
      241 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
      301 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
      361 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
      421 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
      481 cgatcaactc gcgcgtaggt ggacggcgca gaaggggagt agctcttcgc cggaccgtcg
      541 acatactgct cagctcgtcg tcgccaaatg gatccgagcg ttgatccggt aaaaccggat
      601 tcggggaata gccaagagca gtgccttgca gaagttctcg ggcgagaatc tcgcgtagac
      661 acatacatga gcgtttgtaa aggagactga taatggcatg tacaactata ctcgtcggta
      721 aaaaggcatc ttatcggtag tcccggtcgc ctctctcgac gaaccgctta catgcctagc
      781 cgataagcat tatttaggca ctacaactag tatagttgta catgaaaaac gctgtatttt
      841 tttatccatt tccaaagagc gcccaacgcg acctgaaatt tgaataagac tgctgcttgt
      901 gtaaaggcga tgattgttgt gaacattaga ggtcgtatcc tatcgcgata attccatatc
      961 gtacaaaacg aattacaaat tatttgaccg ctaattcaaa tcaagggttg aaaactttgt
     1021 aatttttgga gcgccggatc agggagtgga cggcctggga gcgctacacg ctgtggctgc
     1081 ggtcggtgca aaaagatttg atgcccgctg cgcacatcta gttaatgtcc gcaagactcc
     1141 agatcagggt gcgcaagttg tcaacgctcc caggagagtt atcgacttgc gtattaggga
     1201 gacgagaaac gttccgtccg tctgggtcag ttgggcaaag ttgatgaccg ggtcgtccgt
     1261 tccttatcgg tgtaggtcga ttttggacag aaatatatgg ggccagtaag cttacaagct
     1321 gaacctgcgt aaaagttaag gttaggcatg taaacttaac tctcacacag gtttataaca
     1381 cccaatcatc agataactat ggcggcacgt gcattaacca cggttgtatc ccgtctaaag
     1441 tactcgtgcg ttggtatact tcagtcttaa ccaatgcacc ggtttgtgcg gatgcctatt
     1501 ttattatgga agtttgttca ctcaacattg caagactgta catacttcca tagtttatta
     1561 atttatattg cgaaaaataa ttggcgaacg aggtaactgg atacctcatc cgccaattaa
     1621 aatttgagca ccattgcctt ggcgatcagc atgaggctgc ctgagatcct ctagaataat
     1681 tggaagttcg gaataactat gcagatacct gatacacact tccaacaaaa acaaccacaa
     1741 gcacgctgat aatcagcaag accaccaaca tttccaccaa tgtaaaagct ttaaccttag
     1801 cttatacaca aatctaattt tccatgtagg ctgcacgacc ccgtccaata gtcaaaagag
     1861 tgagagtttt actatccttg attgagatgt aggttactaa aattatttat attttccaaa
     1921 tggattttgc agattcccag atgcccctac agaaagaggt acaaaacatt tattggaatt
     1981 aattagttcg tgttttggat tgttacacgt tctctagctt atgatctgag cgtaagttga
     2041 aatatgttta ctaataagac tttatgggtt gagagcgtct tattagtaaa catataaacg
     2101 ttcgtggtaa ctatgggtgg tacaggtgcc acattagttg taccatttat gtttatgtgg
     2161 ttaacccaga ggaacactcc gtcggtcgcg cactaggaag aattccgaag gtcccttaaa
     2221 ttttaatagc agttgtgtca ctatttaggt ctacccagtg acacaactaa catacaaaac
     2281 aataacggtt gtatttgtag aacttgacca gttgttttag taacataaat acaactccga
     2341 atactcatag ccccccgatt cgccaggtac ttacgaggaa ttacttatat ccgactcaca
     2401 tggattgctt atatttacag gaccaactgg atctggaaaa acaacaacaa tgtatgcgcg
     2461 ctagacctca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     2521 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     2581 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     2641 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     2701 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     2761 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     2821 gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     2881 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     2941 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     3001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     3061 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     3121 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     3181 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     3241 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaag
     3301 gggtctgacg ctcagtggaa cgaaaagtat atatgagtaa acttggtctg acagctcgag
     3361 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     3421 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     3481 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     3541 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     3601 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     3661 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     3721 tctcaataaa ccctttaggg aaataggcca ggttttcac
//
