LOCUS       sequence-97269-e         372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of dsRed and GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW
  TITLE     Dynamics of intracellular bacterial replication at the single cell 
            level.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 
            10.1073/pnas.1000041107. Epub 2010 Feb 2.
  PUBMED    20133586
REFERENCE   2  (bases 1 to 372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1000041107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-02-23- 23"; volume: "107"; issue: "8"; pages: "3746-51"
COMMENT     Sequence Label: pDiGi
FEATURES             Location/Qualifiers
     source          1..372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(177..195)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(227..247)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 accatggtac ccggggatcc tctagattta agaaggagat atacatatgg cctccaccga
       61 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
      121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      181 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
      241 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
      301 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcaaggacg gcggcgtggt
      361 gaccgtgacc ca
//
