LOCUS       sequence-97283-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive expression of T7 sigma fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_44
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(349..398)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    456..477
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      480..523
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(527..545)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtatcttcc agcacttgtt gacggaagcc gaactctttg gacccgtaag ccagcgtcat
       61 gactgaactc ttagtcacac tgcgagtaac accgtaagcc agccattgac cagccagtgc
      121 cttagtgccc agcttgactt tctcagagat ttcaccagtg ttcttaccac ctgaacctcc
      181 gaagtacgct tcttcacgct ccagtttcgc caggtcacgt tccaggttcg cgatgtcttt
      241 ttcgagacga gcgttttcgt tttccagacg cgccagatcg ttctccaggg tcgccgcgat
      301 gctcatctag tactttcctg tgtgattaaa caaaattatt tgtagaggct gtttcgtcct
      361 cacggactca tcagaccgga aagcacatcc ggtgacagaa acatagtcca tagactagta
      421 gctagcctat agcctaggac tgagctagct gtcaactcta gaagcggccg cgaattccag
      481 aaatcatcct tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc
      541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactcga
      601 gttgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
      661 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaa
//
