LOCUS       sequence-97288-e       10614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a 4-integrase plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pCis_2+7+8+5
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..84
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(580..599)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             591..1406
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             591..1406
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(664..683)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1523..1540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      2114..2702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2603..2622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3184..3213)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(3260..4138)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4165..4449
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4372..4391
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 acttccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
       61 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      121 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      181 atttaacgcg aattttaaca aaatacttca ctgacaccct catcagtgcc aacatagtaa
      241 gccagtatac actccgctag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat
      301 accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag
      361 ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg
      421 cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac
      481 aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
      541 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      601 ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat
      661 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
      721 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      781 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      841 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggga
      901 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      961 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     1021 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     1081 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     1141 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     1201 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1261 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1321 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1381 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     1441 gcattacgct gacttgacgg gactcgagat taccgccttt gagtgagctg ataccgctcg
     1501 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca taacgcgaag
     1561 taatcttttc ggttttaaag aaaaagggca gggtggtgac accttgcccg tttttttgcc
     1621 ggactgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2221 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga ggtcggcgat ataggcgcca
     2881 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc
     2941 tcatgtttga cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat
     3001 tctgcaaacc ctatgctact ccctcgagcc gtcaattgtc tgattcgtta ccaattatac
     3061 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     3121 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg
     3181 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt
     3241 agttagccct tagtgactct tatgacaact tgacggctac atcattcact ttttcttcac
     3301 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     3361 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     3421 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     3481 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     3541 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     3601 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     3661 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     3721 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     3781 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     3841 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     3901 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     3961 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     4021 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     4081 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     4141 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     4201 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     4261 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     4321 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     4381 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     4441 tttctccata cccgtttttt tgggctagca acatagcgaa tcgtaaggag ttaaaagatg
     4501 ccgattgcac cggaatttct gagcctggca tatccgggtc aagaatttcc ggcatatctg
     4561 tatggtcgtg caagccgtga tccgaaacgt aaaggtcgta gcgttcagag ccagctggat
     4621 gaaggtcgtg ccacctgtct ggatgcaggt tggccgattg ccggtgaatt taaagatgtt
     4681 gatcgtagcg caagcgcata tgcacgtcgt acccgtgatg aatttgaaga aatgattgca
     4741 ggtattcagg caggcgaatg tcgtattctg gttgcatttg aagcaagccg ttattatcgt
     4801 gatctggaag catatgttcg tctgcgtcgt gtttgtcgtg aagccggtgt tctgctgtgt
     4861 tataatggtc aggtttatga tctgagcaaa agcgcagatc gtaaagcaac cgcacaggat
     4921 gcagttaatg ccgaaggtga agcagatgat attcgtgaac gtaatctgcg taccacccgt
     4981 ctgaatgcaa aacgtggtgg tgcacatggt ccggttccgg atggttataa acgtcgttat
     5041 gatccggata gcggtgatct ggttgatcag attccgcatc ctgatcgtgc aggtctgatt
     5101 accgaaattt ttcgtcgtgc agcagcagcc gaaccgctgg cagcaatttg ccgtgatctg
     5161 aatgaacgtg gtgaaaccac ccatcgtggt aaagcatggc agcgtcatca tctgcatgca
     5221 attctgcgta atcctgcata tattggtcat cgtcgtcatc tgggtgttga taccggtaaa
     5281 ggtatgtggg caccgatttg tgatgatgaa gattttgcag aaacctttca ggccgttcaa
     5341 gaaattctga gtctgcctgg tcgtcagctg agtccgggtc cggaagcaca gcatctgcag
     5401 accggtattg cactgtgtgg tgaacatcct gatgaaccgc ctctgcgtag cgttaccgtt
     5461 cgtggtcgta ccaattataa ctgtagcacc cgttatgatg ttgccatgcg tgaagatcgt
     5521 atggatgcat ttgttgaaga aagcgtgatt acctggctgg caagtgatga agcagttgca
     5581 gcctttgaag ataacaccga tgatgaacgt acccgtaaag cacgtattcg tctgaaagtt
     5641 ctggaagaac agctggaagc agcacagaaa caggcacgta ccctgcgtcc ggatggcatg
     5701 ggtatgctgc tgagcattga tagcctggca ggtctggaag ccgaactgac accgcagatt
     5761 gataaagccc gtcaagaaag ccgtagcctg catgttccgg cactgctgcg tgatctgctg
     5821 ggtaaaccgc gtgcagatgt ggatcgtgca tggaatgaag cactgaccct gccgcagcgt
     5881 cgcatgattc tgcgcatggt tgttaccatt cgtctgttta aagcaggtag ccgtggtgtt
     5941 cgtgcaattg aaccgggtcg tattaccctg agctatgttg gcgaaccggg ttttaaaccg
     6001 gttggtggta atcgtgcaaa acagtaatta gctgaagtaa tttcaacaaa ataactagga
     6061 ttcgaatgaa agtggccatt tatgttcgtg ttagcaccga tgaacaggcc aaagaaggtt
     6121 ttagcattcc ggcacagcgt gaacgtctgc gtgcattttg tgcaagccag ggttgggaaa
     6181 ttgtgcaaga atatattgaa gaaggttgga gcgcaaaaga tctggatcgt ccgcagatgc
     6241 agcgtctgct gaaagatatc aaaaaaggca acattgatat tgtgctggtg tatcgtctgg
     6301 atcgcctgac ccgtagcgtt ctggatctgt atctgctgct gcagaccttt gaaaaataca
     6361 atgtggcatt tcgtagcgcc accgaagttt atgataccag caccgcaatg ggtcgtctgt
     6421 ttattaccct ggttgcagca ctggcacagt gggaacgtga aaatctggca gaacgtgtta
     6481 aatttggtat cgagcagatg atcgatgaag gtaaaaaacc gggtggtcat agcccgtatg
     6541 gttacaaatt tgataaagac ttcaattgca ccattattga ggaagaagca gacgttgttc
     6601 gtatgatcta tcgcatgtat tgtgatggtt atggctatcg tagcattgca gatcgtctga
     6661 atgaactgat ggttaaaccg cgtattgcca aagaatggaa tcataatagc gtgcgtgata
     6721 tcctgaccaa cgatatctat attggcacct atcgttgggg tgataaagtt gttccgaata
     6781 atcatccgcc tattattagc gaaaccctgt tcaaaaaagc ccagaaagaa aaagaaaaac
     6841 gtggcgttga tcgtaaacgc gttggtaaat ttctgtttac cggtctgctg cagtgtggta
     6901 attgtggtgg ccataaaatg cagggccatt ttgataaacg tgagcagaaa acctattacc
     6961 gttgtaccaa atgtcaccgc attaccaacg aaaaaaacat tctggaaccg ctgctggatg
     7021 aaattcagct gctgattacc agcaaagaat actttatgag caaattcagc gaccgctatg
     7081 atcagcaaga ggttgttgat gttagcgcac tgacaaaaga actggaaaaa atcaaacgcc
     7141 agaaagagaa atggtacgat ctgtatatgg atgatcgtaa cccgattccg aaagaagaac
     7201 tgtttgccaa aattaacgaa ctgaacaaaa aagaagaaga aatctatagc aagctgagcg
     7261 aagtggaaga agataaagaa ccggttgaag agaaatataa ccgcctgagc aaaatgatcg
     7321 attttaaaca gcagtttgag caggccaacg actttaccaa aaaagagctg ctgttcagca
     7381 tcttcgaaaa gattgtgatt tatcgcgaga aaggcaagct gaaaaaaatc accctggatt
     7441 acaccctgaa ataagaataa aacaataact aggattcgaa tgaaagttgc cgtttattgt
     7501 cgtgttagca ccctggaaca gaaagaacat ggtcatagca ttgaagaaca agagcgtaaa
     7561 ctgaaaagct tctgcgatat taatgattgg accgtgtatg atacctatat cgatgcaggt
     7621 tatagcggtg caaaacgtga tcgtccggaa ctgcagcgtc tgatgaatga tattaacaaa
     7681 tttgatctgg tgctggtgta taaactggat cgtctgaccc gtaatgttcg tgatctgctg
     7741 gacctgctgg aaatctttga aaaaaatgat gtgagctttc gtagcgccac cgaagtttat
     7801 gataccacca ccgcaatggg tcgtctgttt gttaccctgg ttggtgcaat ggcagaatgg
     7861 gaacgtgaaa ccattcgtga acgtacccag atgggtaaac tggcagcact gcgtaaaggt
     7921 attatgctga ccacccctcc gttttattat gaccgtgtgg ataataagtt tgtgccgaac
     7981 aaatacaaag acgttattct gtgggcatat gacgaagcaa tgaaaggtca gagcgcaaaa
     8041 gcaattgcac gcaaactgaa taatagcgat attccgcctc cgaataatac ccagtggcag
     8101 ggtcgtacca ttacccatgc cctgcgtaat ccgtttaccc gtggtcattt tgattggggt
     8161 ggtgtgcata ttgaaaataa ccatgaaccg atcatcaccg atgagatgta tgagaaagtt
     8221 aaagatcgcc tgaatgaacg cgtgaacacc aaaaaagttc gtcataccag catttttcgt
     8281 ggcaaactgg tttgtccggt ttgtaatgca cgcctgaccc tgaatagcca taaaaagaaa
     8341 agcaatagcg gctatatctt tgtgaaacag tactactgca acaactgtaa agttaccccg
     8401 aatctgaaac cggtgtacat caaagaaaaa gaagtgatta aagtttttta caattatctg
     8461 aaacgcttcg atctggaaaa atatgaggtt acccagaaac agaacgaacc ggaaatcacc
     8521 atcgatatca ataaagttat ggaacagcgc aaacgctacc ataaactgta tgcaagcggt
     8581 ctgatgcaag aagatgaact gtttgacctg attaaagaaa ccgatcagac cattgccgaa
     8641 tatgaaaaac agaatgaaaa ccgcgaagtg aagcagtatg atatcgaaga tatcaaacag
     8701 tataaagatc tgctgttaga aatgtgggat atcagctccg atgaagataa agaggacttt
     8761 atcaaaatgg cgattaaaaa catctatttt gaatatatca ttggcaccgg taacaccagc
     8821 cgtaaacgta atagcctgaa aattacgagc attgaattct attaagaatc agaggaagga
     8881 ggctcgatgc ctggtatgac caccgaaacc ggtccggatc ctgcaggtct gattgacctg
     8941 ttttgtcgta aaagcaaagc agttaaaagc cgtgcaaatg gtgcaggtca gcgtcgtaaa
     9001 caagaaatta gcattgcagc acaagaaacc ctgggtcgta aagttgcagc actgctgggt
     9061 atgcaggttc gtcatgtttg gaaagaagtt ggtagcgcaa gccgttttcg taaaggtaaa
     9121 gcacgtgatg atcagagcaa agcactgaaa gccctggaaa gcggtgaagt tggtgcactg
     9181 tggtgttatc gtctggatcg ttgggatcgt ggtggtgccg gtgcaattct gaaaattatc
     9241 gaaccggaag atggtatgcc tcgtcgtctg ctgtttggtt gggatgaaga taccggtcgt
     9301 ccggttctgg atagcaccaa taaacgtgat cgcggtgaac tgattcgtcg tgcagaagaa
     9361 gcacgcgaag aagcagaaaa actgagcgaa cgtgttcgtg ataccaaagc acatcagcgt
     9421 gaaaatggtg aatgggttaa tgcccgtgca ccgtatggtc tgcgtgttgt tctggttacc
     9481 gttagtgatg aagagggtga tgaatatgat gaacgtaaac tggcagcaga tgatgaagat
     9541 gcgggtggtc ctgatggtct gaccaaagca gaagcagccc gtctggtttt taccctgccg
     9601 gttaccgatc gtctgagcta tgcaggcacc gcacatgcaa tgaatacccg tgaaattccg
     9661 agcccgaccg gtggtccgtg gattgcagtt accgtgcgtg atatgattca gaatccggca
     9721 tatgcgggtt ggcagaccac aggtcgtcag gatggtaaac agcgtcgtct gaccttttat
     9781 aacggtgaag gtaaacgtgt tagcgttatg catggtcctc cgctggtgac cgatgaagaa
     9841 caagaagccg caaaagcagc cgttaaaggt gaagatggtg ttggtgttcc gctggatggt
     9901 agcgatcatg atacccgtcg caaacatctg ctgagcggtc gtatgcgttg tccgggttgt
     9961 ggtggtagct gtagctatag cggtaatggt tatcgttgtt ggcgtagcag tgtgaaaggt
    10021 ggttgtccgg caccgaccta tgttgcacgt aaaagcgttg aagaatatgt tgcatttcgt
    10081 tgggcagcaa aattagcagc aagcgaaccg gatgatccgt ttgttattgc agttgcagat
    10141 cgctgggcag cactgaccca tccgcaggca agcgaagatg aaaagtatgc aaaagccgca
    10201 gttcgtgaag ccgaaaaaaa tctgggtcgc ctgctgcgtg atcgtcagaa tggtgtttat
    10261 gatggtccgg cagaacagtt ttttgcccct gcatatcaag aagcactgag caccctgcag
    10321 gcagccaaag atgcagttag cgaaagcagc gcaagcgcag cagttgatgt tagctggatt
    10381 gttgatagca gcgattatga agaactgtgg ctgcgtgcaa ccccgaccat gcgtaatgca
    10441 attattgata cctgcatcga tgaaatttgg gttgcaaaag gccagcgtgg tcgtccgttt
    10501 gatggtgatg aacgcgttaa aatcaaatgg gcagcccgta cctaacagat aaaaaaaatc
    10561 cttagctttc gctaaggatg atttctgatt agggtgatgg ttcacgtagt gggc
//
