LOCUS       sequence-97294-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with mCherry ectodomain, no linker domain, A 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: mCherry_LD 0_CS A_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     277..296
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(370..390)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     primer_bind     complement(744..763)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
       61 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
      121 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
      181 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
      241 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
      301 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
      361 tctagacatt gcctgaaaat acaggttttc aacacaaaga gccactgtca ctagcaagcc
      421 ataacaaaac aggactccag caaccacgac cagtgcccaa aaaagcttct tgtacagctc
      481 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta
      541 gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg
      601 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt
      661 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
      721 ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
      781 ggtgccgcgc agcttcacct tgtagatg
//
