LOCUS       sequence-97296-e         327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human UBE2D3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-UBE2D3i2
FEATURES             Location/Qualifiers
     source          1..327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(104..137)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     141..161
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc
       61 tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat
      121 acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt
      181 gtgttttgag actataaata tcccttggag aaaagccttg tttgatgcca tgtgatgcta
      241 ccttaattct agagattaag gtagcatcac atggcatctt ttttctcgag tactaggatc
      301 cattaggcgg ccgcgtggat aaccgta
//
