LOCUS       sequence-97298-e         523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human UBE2E3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-UBE2E3i1
FEATURES             Location/Qualifiers
     source          1..523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..136)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     140..160
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
       61 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
      121 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
      181 tgttttgaga ctataaatat cccttggaga aaagccttgt ttgttcacat aatttgtatg
      241 cagtgttcta gagacactgc atacaaatta tgtgaacttt tttctcgagt actaggatcc
      301 attaggcggc cgcgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
      361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      481 ttcccatagt aacgccaata gggactttcc attgacgtca atg
//
