LOCUS       sequence-97307-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) 
            cDNA allele E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   McBride CS, Baier F, Omondi AB, Spitzer SA, Lutomiah J, Sang R, 
            Ignell R, Vosshall LB
  TITLE     Evolution of mosquito preference for humans linked to an odorant 
            receptor.
  JOURNAL   Nature. 2014 Nov 13;515(7526):222-7. doi: 10.1038/nature13964.
  PUBMED    25391959
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-11-13- 13"; volume: "515"; issue: "7526"; 
            pages: "222-7"
COMMENT     Sequence Label: pUASTattb AaegOr4-E
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    162..195
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    228..322
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        341..579
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     526..547
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt
       61 gggcataata gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt
      121 taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg tataatgtat
      181 gctatacgaa gttatgctag cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc
      241 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
      301 gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata aatagaggcg
      361 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
      421 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
      481 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
      541 tgccaagaag taattattga atacaagaag agaactctga atagggaatt gggaattcat
      601 gactcgatcc atcgagtttg atcaaacctt taggtttatc gctaaagtac ttcaaatgat
      661 cggttaccca agctgtatag caccataccc aacgaccttc gccagtcgac ttaagtccag
      721 tgccggtttc gtggtatgct tcctcatgtt gacctattgc gttttcggtc agatcatcaa
      781 catcggcctg ctgatgatgg gttaccgcca gacggatcag atcgtcgagg aggtcgccat
      841 ccaggtcagc agcacgggct tctgcatcat cggactggcg aagatgtaca gtttgtcgta
      901 caaccgggcc attctgagct ggctcattgc ggacttcaga gtcaagtgga atgccgggga
      961 gctcacagag aaggaccgtt acatacggga tgggacgtta cgtccaacgg tggccattac
     1021 gactgtagcg gcgctaggga acatcatcat ggtgtcggcg ttcaactttc aaccggta
//
