LOCUS       sequence-97310-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with mCherry ectodomain, no linker domain, M 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: mCherry_LD 0_CS M_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     279..298
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(372..392)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
ORIGIN
        1 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
       61 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      121 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      181 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      241 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      301 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
      361 aatctagaca tcatctgaaa atacaggttt tcaacacaaa gagccactgt cactagcaag
      421 ccataacaaa acaggactcc agcaaccacg accagtgccc aaaaaagctt cttgtacagc
      481 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
      541 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
      601 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
      661 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      721 tcggaggagg cctcccagcc catggtcttc ttctgcatta c
//
