LOCUS       sequence-97323-e         298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human ADAM21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-ADAM21i1
FEATURES             Location/Qualifiers
     source          1..298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..141)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     145..165
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga cagataacct
       61 gttcttttta atactagcta cattttacat gataggcttg gatttctata acttcgtata
      121 gcatacatta tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
      181 ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgttaacg cgcggtgacc
      241 ctcgaggcag tctgtctgtc ttgtcacatt tctagagaat gtgacaagac agacagac
//
