LOCUS       sequence-97324-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201N Cas9
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(6..258)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(325..345)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
       61 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
      121 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
      181 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
      241 ccaaatgttt gaacgatcgg ggaaattcga gctcggccgg cctcgagacg tcgacgcgta
      301 agcttctgca gatatccgcg gtcacacctt cctcttcttc ttggggtcag ccctgctgtc
      361 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat
      421 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc
      481 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt
      541 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc
      601 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc
      661 atcaaggtag tg
//
