LOCUS       sequence-97326-e         404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV based expression vector - expresses GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 404)
  AUTHORS   So AY, Sookram R, Chaudhuri AA, Minisandram A, Cheng D, Xie C, Lim 
            EL, Flores YG, Jiang S, Kim JT, Keown C, Ramakrishnan P, Baltimore D
  TITLE     Dual mechanisms by which miR-125b represses IRF4 to induce myeloid 
            and B-cell leukemias.
  JOURNAL   Blood. 2014 Aug 28;124(9):1502-12. doi: 
            10.1182/blood-2014-02-553842. Epub 2014 Jul 8.
  PUBMED    25006123
REFERENCE   2  (bases 1 to 404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-02-553842"; journalName: "Blood"; date: 
            "2014-08-28- 28"; volume: "124"; issue: "9"; pages: "1502-12"
COMMENT     Sequence Label: pMG
FEATURES             Location/Qualifiers
     source          1..404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..55)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(38..55)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(74..90)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(74..90)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(87..109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    98..114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(122..128,129..146,147..152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(305..322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtgtcacc taaataggct
       61 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      181 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      241 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taac
//
