LOCUS       sequence-97333-e         536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to 
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201G Cas9
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(276..292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(289..311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(324..330,331..348,349..354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    369..390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
       61 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
      121 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
      181 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattga tttaattaag
      241 ccgcgcctac cctctagtca aggattcgta atcatgtcat agctgtttcc tgtgtgaaat
      301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      481 ttgcgtattg gctagagcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaa
//
