LOCUS       sequence-97342-e         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA against human PTPRK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust 
            EM, McManus MT, Song JS, Ramalho-Santos M
  TITLE     Systematic Identification of Barriers to Human iPSC Generation.
  JOURNAL   Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040.
  PUBMED    25036638
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.05"; journalName: "Cell"; date: "2014-07-17- 
            17"; volume: "158"; issue: "2"; pages: "449-61"
COMMENT     Sequence Label: pSicoR-PTPRKi1
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..141)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     145..165
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gcatataata tttcctagta actatagcag gcttaatgtg cgataaaaga cagataatct
       61 gttcttttta atactagcta cattttacat gataggcttg gatttctata acttcgtata
      121 gcatacatta tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa
      181 ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgtttgca gagaccaagt
      241 cattacattg ttctagagac aatgtaatga cttggtctct gcttttttct cgagtactag
      301 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcattagt
//
