LOCUS       sequence-97358-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for homologous recombination in mouse ES cells; 
            Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        265..764
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(300..323)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     697..716
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tttaattcag tcagtagtct taagtggtct ttattggccc ttcattaaaa tctactgttc
       61 actctaacag aggctgttgg tactagtggc acttaagcaa cttcctacgg atatactagc
      121 agattaaggg tcagggatag aaactagtct agcgttttgt atacctacca gctttatact
      181 accttgttct gatagaaata tttcaggaca tctagcttat cgataccgtc gacggtatcg
      241 ataagcttga tatcgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
      301 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
      361 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
      421 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
      481 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
      541 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
      601 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
      661 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
      721 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc a
//
